Accurate non-invasive image-based cytotoxicity assays for cultured cells
نویسندگان
چکیده
BACKGROUND The CloneSelect Imager system is an image-based visualisation system for cell growth assessment. Traditionally cell proliferation is measured with the colorimetric MTT assay. RESULTS Here we show that both the CloneSelect Imager and the MTT approach result in comparable EC50 values when assaying the cytotoxicity of cisplatin and oxaliplatin on various cell lines. However, the image-based technique was found non-invasive, considerably quicker and more accurate than the MTT assay. CONCLUSIONS This new image-based technique has the potential to replace the cumbersome MTT assay when fast, unbiased and high-throughput cytotoxicity assays are requested.
منابع مشابه
A Comparison between the Cytotoxicity Induced by Gossypol in Two Testicular Cell Lines
Background:Gossypol is a yellow toxic pigment from the cottonseed that can cause acute or chronic toxicity in humans and animals by affecting the testicular tissues. Nowadays cottonseed is used as food supplement for ruminants specially the sheep. In this study, two different stem cell lines of testicular tissue including GC1-spg (mouse testis) and SFTF-PI43 (sheep testis) cells were used to ev...
متن کاملNon-invasive determination of cellular oxygen consumption as novel cytotoxicity assay for nanomaterials.
Investigating the safety of nanoparticles is essential for many fields of their applications, in particular for consumer products, food and medicines. The conventional dye and fluorescence-based cytotoxicity assays are limited by the interference of such readouts with nanomaterials. This holds in particular when nanomaterials have been fluorescently labelled for other purposes, for example, con...
متن کاملI-1: Screening of Subfertile Men for Testicularlar Carcinoma In Situ by An Automated Image Analysis-Based Cytological Test of The Ejaculate
Background: Testicular cancer (TC) is usually diagnosed after manifestation of an overt tumour. Tumour formation is preceded by a pre-invasive and asymptomatic stage, carcinoma in situ (CIS) testis, except for very rare subtypes. The CIS cells are located within seminiferous tubules but can be exfoliated and detected in ejaculates with specific CIS markers. Materials and Methods: We have built ...
متن کاملAn integrated approach to improved toxicity prediction for the safety assessment during preclinical drug development using Hep G2 cells.
Efficient and accurate safety assessment of compounds is extremely important in the preclinical development of drugs especially when hepatotoxicity is in question. Multiparameter and time resolved assays are expected to greatly improve the prediction of toxicity by assessing complex mechanisms of toxicity. An integrated approach is presented in which Hep G2 cells and primary rat hepatocytes are...
متن کاملSensitivity of Hematopoietic Malignant Cells to Peganum Harmala Seed Extract in Vitro
Background and Objective: Peganum harmala is a medicinal plant that has been used for treatment of numerous diseases including viral, bacterial and parasitic infections. Anti-proliferative and anti-tumor effects of Peganum harmala extracts and its derivatives have been reported. In the present study, cytotoxic effect of Peganum harmala seeds aquaous extract on leukemic U937 and Molt-4 cells was...
متن کامل